Introduction: Aesthetic dentistry has developed in the recent decades. Orthodontic treatment has become one of the more popular ways for alignment of crowded teeth. However, when braces are removed, white spot lesions (WSLs) may have a detrimental impact on the patient's aesthetics and pleasure. Aim: this research aimed to evaluate the clinical effect of Self assembling peptides and Amorphous calcium phosphate varnish on the treatment of post orthodontic enamel WSLs. Materials and methods: A total of 40 patients teeth were enrolled in this prospective single-blinded randomized clinical study categorized into four groups (10 per group); Control group , Self assembling peptide P11-4 group (Curodont ™ Repair /Regenamel®), Amorphous Calcium Phosphate varnish group (Enamel Pro® Varnish 5% Sodium Fluoride Premier Dental) and combination group in which the two analogues were combined with each other. Enamel white spot lesions were evaluated quantitatively using Diagnodent® (KaVo Dental Corporation, USA). Results: A quantitative increase in remineralization of post orthodontic WSLs in all the groups and over time intervals. However, the WSLs recovery was significantly better in the combination group followed by the self-assembling peptide group, than control group which showed the least values of remineralization. Conclusion: Combining self-assembling peptideP11-4 with other inorganic remineralizing analogues provides additional benefits of accelerating and enhancing the remineralization process, allowing for significantly faster and improved regenerative repair for post orthodontic WSLs.