Introduction: Dentin hypersensitivity is a widespread issue with high prevalence.
Blocking the open dentinal tubules is one way to reduce dentin hypersensitivity.
Aim: The current study aimed at evaluating the in vitro potential of a novel experimental
dentifrice made of nano-fluoro hydroxyapatite (n-FHAP) produced from a biogenic
source (cuttlefishbones) for occlusion of dentinal tubules. Material and Methods: 30
dentin discs (n = 30) were classified into 3 categories (n = 10) based on the dentifrices
they utilized for brushing: 1st category = artificial saliva (AS; control); 2nd category
= fluoride dentifrice (Sensodyne, UK); 3rd category = experimental nano-FHAP dentifrice.
Then, after brushing, discs were subjected to an acidic challenge with 6 percent
wt citric acid (pH = 4.0) for 1 minute. By utilizing a SEM (scanning electron
microscope) and EDX (energy-dispersive X-ray) spectroscopy before and after the
citric acid challenge, the tubule occlusion was measured. Results: Group 1 (AS) SE
micrographs revealed no tubule blockage. The tubules in Groups 2 and 3 were mostly
blocked, according to SE micrographs. Data before and after the citric acid challenge
data were statistically significant (p= 0.05) for all groups. Conclusion: In terms of tubule
blockage, the findings of the new experimental dentifrice were equivalent to those
of Sensodyne. Dentifrice containing FHAP nano-particles might be used to treat dentin
sensitivity as an alternative.