Objectives: the aim of the study was the evaluation of healing in bony defects comparing milled teeth versus beta tricalcium phosphate (B-TCP) as bone graft materials. Materials and methods: Forty-five male albino rats, were divided into three groups each group included 15 rats; one control and two experimental groups. Under general anesthesia, 1.5 cm incision was created to expose the cranial bone. Then two bone defects (each one with a diameter of 3 millimeter) were created one in each parital sides. In Group I (control group): the two bony defects (30 bony defects) were left to heal without any intervention. While, in Group II & III the bony defects were completely filled with (B-TCP) and milled teeth, respectively. At the 7th, 15th, and 30 days postoperatively five rates were randomly selected from each group to be sacrificed. Results: histomorophometric and histological results showed a higher level of bone regeneration in the two experimental groups than that of the control group all over the study period, moreover there was a significant increase in the amount of (newly formed bone) in the milled teeth than in the (B-TCP) group. The remaining particles of the two graft materials was significantly less in the milled teeth group than in the (B-TCP) group during the whole study time. Conclusion: Bone activity, formation and maturity were ahead in tooth ash filled calvarial bony defects.