Two chemical mutagens sodium azid (SA) and hydrazine hydrate (HZ) were used to induce genetic variability for improving some morphological and agronomical traits in three bread wheat genotypes namely, Sids₁, Sids₁₂ and Giza₁₆₈. Some mutations were selected in M₁ generation plants i.e., three for Sids₁ variety )S₁ 29, S₁ 49, and S₁ 75), four for Sids₁₂ variety i.e., (S₁₂ 116, S₁₂ 161, S₁₂ 168 and Sids₁₂ 177) and one for Giza₁₆₈ (G₁₆₈ 202). Selected genotypes were grown in field experiment to obtain M₂ generation. Results showed that most agronomical traits were significantly increased in M₂ than M₁ generation plants. The highest values in selected mutated plants for 100 grain weight were 6.31 gm for S₁₂ 161 plant, 5.79 gm for G₁₆₈ 202 plant and 5.55 gm for S₁ 29 plant. Genetic variation in M₂ plants were used of evaluated by SSR molecular markers. The results showed representable the variation between treated genotypes and untreated (control) for all studied traits.