This study was carried out to determine the frequency of occurrence and the presence of true spider associated with banana, citrus, grape and guava fruit trees. This study was conducted at El-Menshah and Tahta districts at sohag governorate, Egypt during two successive years 2018/2019 and 2019/2020. A total of specimens belonging to 75 species, 48 genera and 32 families were collected. The most dominant family with the largest number of species were Lycosidae, Philodromidae, Theridiidae, Cheiracanthiidae, Salticidae, Gnaphosidae and Thomisidae. While, the families Titanoecidae, Dictynidae, Scytodidae, Hersiliidae, Sparassidae, Corinnidae, Oonopidae,Eresidae, Liocranidae, Pisauridae, Agelenidae and Tetragnathidae including few species. As spiders are potential biological control agents of fruit orchard's pests, so any practice that may disrupt this group should be avoided by the farmers.