The present work was done to elucidate the possible protective and antioxidant effect of chromium (Cr) as chromium chloride (CrCl2; 500 mg/k.g.), selenium (Se) as sodium selenite (Na2SeO3; 1.5 mg/k.g.) and zinc (Zn) as zinc sulphate (ZnSO4; 54 mg/k.g.) supplementation in streptozotocin induced diabetic rats. Administration of these elements daily for 60 days showed a significant decrease in MDA concentrations. The treatment resulted also in a significant increase in plasma insulin C-peptide, blood reduced glutathione (GSH) levels and superoxide dismutase (SOD)activity as well as gene expression of heart glucose transporter 4 (GLUT4) of diabetic rats. Also, the results clearly suggested that the trace elements can normalize effectively the body weight as well as high density lipoprotein (HDL-C) in streptozotocin diabetic rats. The trace elements reduced the risk of diabetic complications through rapid protective effects against lipid peroxidation by scavenging of free radicals there. These effects were more pronounced in Cr- treated group than Se and Zn-treated groups.