The present study investigated the effect of rutin on acrylamide (ACR) toxicity was evaluated in experimental animal rats. The rats were feeding on basal diet and received oral 20 mg/kg b.w of acrylamide with 100, 50 and 25 mg/kg w.t of rutin; others were received the acrylamide concentrations only. The present results showed that 8-hydroxydeoxy guanidine (8-OHdG), protein carbonyl (PC), reactive oxygen species (ROS), glutathione peroxidase (GPx), tumor necrosis factor- (TNF-α), nitric oxide (NO) myeloperoxidase (MPO), caspase-3, bax, P53 and cytochrome-C in liver tissues were increased in rats-treated with acrylamide. This effect of acrylamide was attenuated by rutin. In conclusion, rutin ameliorates hepatotoxicity induced by acrylamide in male albino rats. It can be recommend the use of rutin to mitigate the acrylamide concentration in food products.