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203998

Repopulation of multipotent stem cells derived from adult male rabbits on a polycaprolactone scaffold: an in vitro study

Article

Last updated: 22 Jan 2023

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Abstract

ABSTRACT
Background: MSCs (mesenchymal stem cells) are multipotent, spindle-shaped cells that give them the ability to differentiate into a broad lineage of tissues. Polycaprolactone (PCL) is a synthetic material that has been used several times for the fabrication of scaffolds to be utilized in the field of tissue engineering.
Aim of the work: The main goal of this research is to develop a protocol for isolating, cultivating, and characterizing rabbit bone marrow mesenchymal stem cells (BMSCs), as well as to investigate their capacity to repopulate PCL scaffolds.
Results: In Dulbecco's Modified Eagle's Medium (DMEM) supplied with 10% fetal bovine serum (FBS), BMSCs were successfully isolated, cultivated, and grown. The cells were characterized morphologically by their spindle-shape, processes of cytoplasmic, plastic adhesion tendencies, and formation of colonies. They were characterized by flowcytometric analysis as having a positive CD73 expression and a negative CD45 expression. Before cell seeding, the PCL scaffold was inspected under a scanning electron microscope to assess the PCL material characteristics and pour size, and after cell seeding to detect BMSC attachments to the scaffold.
Conclusion: The protocol used in this investigation was effective for isolation, culture, and characterization of rabbit BMSCs and the cells that were obtained successfully repopulated the PCL scaffold.

DOI

10.21608/aimj.2021.95296.1570

Keywords

Mesenchymal Stem Cells, Polycaprolactone scaffold, Flow cytometry, Cell seeding, Electron microscope

Authors

First Name

Hassan

Last Name

Adly

MiddleName

Ahmed

Affiliation

Pediatric Surgery Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt

Email

dr_hassaneladly@yahoo.com

City

-

Orcid

0000-0001-5954-4801

First Name

Abd El-Wahab

Last Name

Okby

MiddleName

-

Affiliation

Pediatric Surgery Department, Faculty of Medicine, Al-Azhar University Cairo, Egypt.

Email

awy.okby@hotmail.com

City

-

Orcid

-

First Name

Abdel-Aziz

Last Name

Mahmoud

MiddleName

Yehya

Affiliation

Pediatric Surgery Department, Faculty of Medicine, Al-Azhar University Cairo, Egypt.

Email

drabdelazizyehya@hotmail.com

City

-

Orcid

-

First Name

Ahmed

Last Name

El-Shamy

MiddleName

-

Affiliation

Pediatric Surgery Unit, General Surgery Department, Faculty of Medicine, Al-Azhar University (Assiut Branch) Assiut, Egypt

Email

drahmedelshamy2@gmail.com

City

-

Orcid

-

First Name

Rania

Last Name

Galhoom

MiddleName

Abdel-Azim

Affiliation

Human Anatomy and Embryology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt. Tissue culture lab, Center of Excellence of Molecular and Cellular Medicine (CEMCM), faculty of Medicine, Suez Canal University

Email

rania-galhom@hotmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Hashem

MiddleName

A

Affiliation

Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Suez Canal University, 4.5 Ring Road, Ismailia, 41522, Egypt

Email

mohamed.hashem@vet.suez.edu.eg

City

Ismailia

Orcid

0000-0002-0668-2669

First Name

Mahmoud

Last Name

Ahmed

MiddleName

F

Affiliation

Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Suez Canal University, 4.5 Ring Road, Ismailia, 41522, Egypt

Email

mahmoud_ali@vet.suez.edu.eg

City

Ismailia

Orcid

0000-0002-4654-5909

Volume

2

Article Issue

11

Related Issue

29158

Issue Date

2021-11-01

Receive Date

2021-09-22

Publish Date

2021-11-01

Page Start

37

Page End

42

Print ISSN

2682-3381

Online ISSN

2682-339X

Article File

AIMJ_Volume 2_Issue 11_Pages 37-42.pdf

PDF . 506.6kB

Link

https://aimj.journals.ekb.eg/article_203998.html

Detail API

https://aimj.journals.ekb.eg/service?article_code=203998

Order

6

Type

Original Article

Type Code

710

Publication Type

Journal

Publication Title

Al-Azhar International Medical Journal

Publication Link

https://aimj.journals.ekb.eg/

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Details

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Article

Created At

22 Jan 2023