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135145

Evaluation of IL28B Gene Sequence Variations in the Diagnosed Null-Response to DAAs Therapy by Intra PBMCs Nested HCV RNA PCR

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Last updated: 22 Jan 2023

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Abstract

Background: Single nucleotide polymorphisms (SNPs) of the IL-28B gene are reliable predictors of chronic HCV disease course after treatment with Peg-IFN and Ribavirin (RBV).
Aims, Subjects and Methods: Impacts of IL-28B-SNPs on the response to direct acting antivirals (DAAs) were evaluated in posttreatment negative serum and cellular HCV-PCR (group I), solitary intra-PBMCs viral infections (group II), and positive HCV-RNA serology (group III). Twelve weeks after completing DAAs-therapy, 45 patients were recruited and divided into three groups (n=15each), as afro-mentioned. IL-28B gene sequencing was done to detect SNPs in relation to hepatic parenchymal changes in the selected study populations.
Results and conclusion: Changes in liver parenchyma of group III (46.7%) and II (26.6%) were associated with higher HCV-relapse compared to I (P

DOI

10.21608/aimj.2021.40774.1313

Keywords

IL28B gene, PBMCs PCR, DAAs, HCV Relapse

Authors

First Name

Mohamed

Last Name

Abd Alla

MiddleName

Darwish Ahmed

Affiliation

Department of Hepatology, Gastroenterology and Infectious Diseases. Faculty of Medicine, Al-Azhar University

Email

darwish0716@azhar.edu.eg

City

Menouf, Menoufia, Egypt

Orcid

-

First Name

ahmed

Last Name

altambouly

MiddleName

mohammed

Affiliation

hepatology &gastroenterology and infectious diseases alazhar university Cairo

Email

dr_tamboly2014@yahoo.com

City

almansoura

Orcid

-

First Name

Mohamed

Last Name

Abd Alla

MiddleName

Darwish Ahmed

Affiliation

Department of Hepatology, Gastroenterology and Infectious Diseases. Faculty of Medicine, Al-Azhar University

Email

hassanr61@yahoo.com

City

-

Orcid

-

First Name

Galal

Last Name

AbuFarrag

MiddleName

AbdElhameed

Affiliation

Department of Hepatology, Gastroenterology and Infectious Diseases. Faculty of Medicine, Al-Azhar University

Email

drgalal2009@yahoo.com

City

-

Orcid

-

First Name

Islam

Last Name

Ammar

MiddleName

Abdel-Mawla

Affiliation

Department of Hepatology, Gastroenterology and Infectious Diseases. Faculty of Medicine, Al-Azhar University

Email

islamammar2000@gmail.com

City

-

Orcid

-

First Name

Mostafa

Last Name

El-Awady

MiddleName

Kamel

Affiliation

Molecular Genetics- Department of Microbial Biotechnology National Research center – Cairo Egypt

Email

mkawady49@yahoo.com

City

-

Orcid

-

Volume

1

Article Issue

11

Related Issue

19841

Issue Date

2020-11-01

Receive Date

2020-09-13

Publish Date

2020-11-01

Page Start

168

Page End

173

Print ISSN

2682-3381

Online ISSN

2682-339X

Article File

AIMJ_Volume 1_Issue 11_Pages 168-173.pdf

PDF . 632.8kB

Link

https://aimj.journals.ekb.eg/article_135145.html

Detail API

https://aimj.journals.ekb.eg/service?article_code=135145

Order

11

Type

Original Article

Type Code

710

Publication Type

Journal

Publication Title

Al-Azhar International Medical Journal

Publication Link

https://aimj.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023