This study evaluated the mutual function of chickens in the transmission of Campylobacter jejuni and Campylobacter coli to patients in Aswan Governorate, Egypt. Samples from fresh chickens (no= 108) and frozen chickens (no= 100), were collected randomly from supermarkets in Aswan Province, Egypt as well as 60 diarrheal samples were assembled from hospitalized patients. Biochemical and molecular techniques were employed through duplex polymerase chain reaction objecting the 23S rRNA, mapA, and ceuE genes specific to genus Campylobacter, C. jejuni, and C. Coli, respectively, after that virulence genes (flaA and cadF genes) were detected. By using conventional and duplex PCR , the overall incidence of Campylobacter was 29% and 25.4 %, respectively. C. jejuni and C. coli by conventional and PCR were identified as 18.1, 5.1%, and 12.3, 7.2%, respectively, while 5.8% mixed infection was discovered by both techniques. Campylobacter species isolated from 66.7, 25, 17.5 and 18.3% of fresh chickens, frozen chickens, frozen liver and gizzard, and human, respectively with statistically significant difference. Epidemiologically, the insignificant age risk factor was statistically reported in this study among patients although Campylobacter was dominant in the 21-35 and 36-50 age groups. Campylobacter incidence was higher among females (33.3%) than males (11.9%). FlaA virulence gene was detected in 10.3% of both C. jejuni and C. coli isolated from chickens but not be detected in human . cadF virulence gene isolated in 20.5, 23.1, 36.4, and 9.1% of C. jejuni and C. coli of chickens′ and human , respectively.