This study detecting Aspergillus fumigatus in salted fish as feseakh and moloha that is being sold in Sohag city markets using culture methods and scanning electron microscope (SEM). In addition, study the effect of chitosan nano particles (CNP) and carvacrol nanoemulsion (CNE) with five concentrations on the isolated strains of A. fumigatus using well diffusion method (WDM) and SEM. Results showed that A. fumigatus was detected in 11(14.7%) and 14 (18.7%) of feseakh and moloha (75 each), respectively. CNP has no effect on A. fumigatus growth at concentrations 0.5%, 1% and 2% and has inhibitory effect at concentrations 5% and 7 %. CNE inhibits A. fumigatus growth at concentrations 0.5%, 1% and 2 and stops its growth at concentrations 5% and 7 %. The effect of both nanomaterials had increased with high concentration. SEM shows degenerative changes on fungus ultrastructure. Therefore, CNP and CNE as antifungal are suitable for food industry to enhance the food safety.