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215972

Isolation and identification of Enterococcus faecium from Meat products with special reference to some virulence and antibiotics resistance factors.

Article

Last updated: 22 Jan 2023

Subjects

-

Tags

Pre-clinical veterinary sciences (Microbiology, Parasitology, Pharmacology, Forensic medicine and Toxicology).

Abstract

Enterococcus faecium is one of the most superbug pathogen which cause many diseases in human which include wound infection and endocarditis. also, this microorganism show high resistant to common antibiotics which used in hospital especially Vancomycin so the purpose of this study is detection E.Faecium from meat products which can be achieved by isolation and identification of E.faecium and detection some virulence gene and antibiotic resistance gene by PCR. Biochemical and molecular studies were performed on seventeen unidentified gram-stain positive, catalase and oxidase negative, Enterococcus-like organisms recovered from hundred meat products samples. The result of Vitek-2 compact system identified the suspected isolates as E. faecium. Antibiotic susceptibility testing of the isolates indicated resistance to most antibiotics, including penicillin, ampicillin, amoxicillin and vancomycin. The molecular analysis indicated the presence of virulence genes such as hyl (30%), ace (30%) and cylA (90%), and antimicrobial resistance genes as followed vanA gene (50%) and blaZ gene (90%). In conclusion meat products considered a good source of infection by vancomycin resistant Enterococcus Faecium (VRE) which have potential risk on human being so hygienic measurement are required during meat processing.

DOI

10.21608/svu.2022.88396.1137

Keywords

Keywords: Meat products, E. faecium, Virulence genes, antimicrobial resistance genes

Authors

First Name

Waleed

Last Name

Younis

MiddleName

-

Affiliation

microbiology department , faculty of veterinary medicine, south valley university

Email

wkhrdr39@gmail.com

City

-

Orcid

-

First Name

Asmaa

Last Name

Mohamed

MiddleName

A

Affiliation

microbiology department , faculty of veterinary medicine, south valley university

Email

asmaaali5252@yahoo.com

City

qena

Orcid

-

First Name

Mohamed

Last Name

Abd Al-azeem

MiddleName

W

Affiliation

microbiology department , faculty of veterinary medicine, south valley university

Email

m_wael@vet.svu.edu.eg

City

qena

Orcid

-

First Name

Hams

Last Name

Mohamed

MiddleName

M.A

Affiliation

microbiology department , faculty of veterinary medicine, south valley university

Email

drhams85@yahoo.com

City

qena

Orcid

-

Volume

5

Article Issue

1

Related Issue

29813

Issue Date

2022-03-01

Receive Date

2021-07-30

Publish Date

2022-03-01

Page Start

1

Page End

16

Print ISSN

2535-1826

Online ISSN

2535-1877

Link

https://svu.journals.ekb.eg/article_215972.html

Detail API

https://svu.journals.ekb.eg/service?article_code=215972

Order

1

Type

Research article

Type Code

712

Publication Type

Journal

Publication Title

SVU-International Journal of Veterinary Sciences

Publication Link

https://svu.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023