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17937

Some Virulence Genes of Pathogenic Enterococci Isolated from Raw Milk and Some Milk Products

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Last updated: 03 Jan 2025

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Abstract

A total of 150 random samples of raw cow milk and some locally manufactured dairy products including yoghurt,Kareish cheese and ice cream were collected from various dairy shops, and supermarkets in Qena city, Egypt. Samples were examined for the presence of Enterococcus spp.  The investigation revealed that 64, 28, 76, 72 and 16 %of the examined raw milk samples, large and small-scale yoghurt, Kareish cheese and ice cream were contaminated with Enterococcus spp., respectively. Isolates were identified as E. faecalis and E. faecium in percentages of (8 &32), (16 &0), (8 &28), (8 &36), and (4 &0) in the examined raw milk samples, large and small-scale yoghurt, Kareish cheese and ice cream, respectively. The obtained isolates were screened for presence of some virulence genes gelE, asa1, esp and cylA using multiplex PCR.  The results indicated that gelE, asa1, esp and cylA were located in 53.9, 76.9, 69.2,and 30.8% of the total E. faecalis isolates and in 46.9, 71.9, 53.1,and 34.3 % of the total E. faecium isolates, respectively.  The asa1 and esp genes were the predominant virulence traits among all investigated enterococci isolates followed by gelE and cylA genes.  Therefore, the results of this study showed that milk and dairy products can play an important role in the spread of Enterococci with virulence potential through the food chain to the human population

DOI

10.21608/svu.2018.17937

Keywords

Enterococcus spp, Milk, Dairy products, PCR, Virulence genes

Authors

First Name

Margret

Last Name

Shafeek

MiddleName

-

Affiliation

Department of Food Hygiene, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt

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Orcid

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First Name

Laila

Last Name

El-Malt

MiddleName

-

Affiliation

Department of Food Hygiene, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt

Email

-

City

-

Orcid

-

First Name

Karima

Last Name

Abdel Hameed

MiddleName

-

Affiliation

Department of Food Hygiene, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt

Email

-

City

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Orcid

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First Name

Mona

Last Name

El-Zamkan

MiddleName

-

Affiliation

Department of Food Hygiene, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt

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City

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Orcid

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Volume

1

Article Issue

1

Related Issue

3752

Issue Date

2018-06-01

Receive Date

2018-06-20

Publish Date

2018-06-01

Page Start

102

Page End

113

Print ISSN

2535-1826

Online ISSN

2535-1877

Article File

SVU_Volume 1_Issue 1_Pages 102-113.pdf

PDF . 337.8kB

Link

https://svu.journals.ekb.eg/article_17937.html

Detail API

https://svu.journals.ekb.eg/service?article_code=17937

Order

9

Type

Research article

Type Code

712

Publication Type

Journal

Publication Title

SVU-International Journal of Veterinary Sciences

Publication Link

https://svu.journals.ekb.eg/

MainTitle

Some Virulence Genes of Pathogenic Enterococci Isolated from Raw Milk and Some Milk Products

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Type

Article

Created At

22 Jan 2023