In post–mitotic tissues such as skeletal muscles there are no significant cell replacements throughout life; the cells sustain local damage through its pool of specified stem cells. Muscle satellite cells are mononuclear cells that remain in a quiescent state till be activated; when they proliferate and fuse with muscle fibers to donate myonuclei, a process necessary for post-embryonic growth, hypertrophy and tissue repair in this post-mitotic tissue.Modern trends in using (autologous) biological agents ininitiation of regenerative process encourage us to evaluate the efficacy of some of these agents.In this study muscle damage was induced by cardiotoxin; followed by administration of biological agents; autologous physiological serum, L-arginine and IGF-1as a stimulating factors of myogenic satellite cells to initiate the myogenic regenerative process.Our study refers to variable degrees of efficacy in stimulation of myogenic stem cells differentiation and the outcome of the regenerative process. Although the physiological serum had marked ability to stimulate MSC but high number of newly formed myotubules are split. L-arginine despite had delayed regenerating time, but more organization of newly formed myotubules was retained. IGF-1 had rapid effect but associated with considerable number of newly formed split myotubules.We could conclude that for perfect regenerative myogenic tissue not main just formation muscle fibers, but theses fibers must be organized and functional. Healthy functional regenerative process need synchronization of several agents should be considered