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17918

Liver Regeneration and New Technical Possibilities by Two-photon Based Intravital Imaging

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Last updated: 03 Jan 2025

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Abstract

One of the outstanding features of the liver is its enormous regeneration capacity. Compared to other solid organs, such as kidney, heartor brain, the liver shows a superior capacity to regenerate. Probably, this regeneration capacity has evolved during ‘animal plant warfare', when plants protected themselves from herbivores by new toxins and herbivores responded by novel detoxifying enzymes and efficient hepatic regeneration. Control mechanisms of liver regeneration have attracted scientists since decades. One limitation that has hampered progress is the lack of possibilities ofreal-time observations of cellular and subcellular processes in the regenerating liver without removing the organ for analysis. This has now become possible by the introduction of an improved technology of two-photonbased intravital imaging. This technology allows the possibility to perform real-time imaging of theintact liver in anesthetized mice. Resolution is close to the theoretically possible 200 nm and therefore allows imaging of organelles and vesicles. Also, imaging of fast processes in the millisecond range is possible. Using available fluorescent reportermouse systems, it is possible to visualize all resident cell types of the liver, such as hepatocytes, Kupffer cells, stellate cells and sinusoidal endothelial cells. Furthermore, infiltrating immune cells can be imaged during liver injury and regenerationusing cell-specific antibodies or reporter mice. This minireview presents some of the possibilities of intravital imaging and its applicability for research in the field of liver regeneration

DOI

10.21608/svu.2018.17918

Authors

First Name

Ahmed

Last Name

Ghallab

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Affiliation

Leibniz Research Centre for Working Environment and Human Factors at the Technical University Dortmund, Dortmund, Germany.&Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt.

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First Name

Jan

Last Name

Hengstler

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Affiliation

Leibniz Research Centre for Working Environment and Human Factors at the Technical University Dortmund, Dortmund, Germany.

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Volume

1

Article Issue

1

Related Issue

3752

Issue Date

2018-06-01

Receive Date

2018-06-03

Publish Date

2018-06-01

Page Start

4

Page End

15

Print ISSN

2535-1826

Online ISSN

2535-1877

Article File

SVU_Volume 1_Issue 1_Pages 4-15.pdf

PDF . 1.5MB

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https://svu.journals.ekb.eg/article_17918.html

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https://svu.journals.ekb.eg/service?article_code=17918

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Review Article

Type Code

705

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Journal

Publication Title

SVU-International Journal of Veterinary Sciences

Publication Link

https://svu.journals.ekb.eg/

MainTitle

Liver Regeneration and New Technical Possibilities by Two-photon Based Intravital Imaging

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Article

Created At

22 Jan 2023