A set of experiments was done at the tissue culture laboratory, Faculty of Agriculture. Saba Basha, Alexandria. University., during the years of 2021 and 2022 to achieve an optimal and reliable system for micropropagation of Aglaonema ‘Lady Valentine'. Different concentrations of naphthalene acetic acid (NAA;0,0.25,0.5 and1.0 mg/l), benzyladenine (BA; 0, 1, 3, and 5 mg/l), and thidiazuron (TDZ) 0, 0.5, 1.0 and 2.0 mg/l were used for shoot regeneration on the multiplication stage. On the rooting stage, naphthalene acetic acid (NAA) 0, 1.0 and 2.0 mg/l, and indole3-butyric acid (IBA; 0, 0.5, 1.0 and 2.0 mg/l) were used on the medium. The highest shoot proliferation (4.98 & 6.15 cm) was obtained on Murashige and Skoog (MS) medium supplemented with 2.0 mg/l TDZ or 5mg/l BA and 0.5mg/l NAA, respectively.  In vitro rooting was facilitated by using an MS medium supplemented with a high concentration of NAA and IBA   and the highest number of roots (12.0) was obtained by the addition of 1.0 mg/l NAA and IBA to the medium. Regenerated plantlets were acclimatized in the greenhouse with a 100% survival rate.