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265534

Assessment of Anti-inflammatory Activity of Prunus dulcis [Miller D.A. Webb (ALMOND)] SEED Aqueous Extract and Fractions

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Last updated: 03 Jan 2025

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Abstract

Almonds (Prunus dulcis) seeds, from the Rosaceae family, have been reported to contain essential nutrients that reduced inflammation by lowering certain inflammatory markers. This study evaluated the anti-inflammatory activity of P.  dulcis (Almond seed) extract and fractions (n-hexane and aqueous). Dried almond seed (700 g) was pulverized and soaked in distilled water for 24 hrs. The aqueous extract was filtered and the filtrate was lyophilized to obtain the seed aqueous crude extract. The crude extract portion was dissolved in water, partitioned with n-hexane and separately concentrated to dryness to obtain the aqueous and n-hexane fractions. Anti-inflammatory activity of almond seed extract and fractions were carried out via erythrocyte membrane stabilization, antitryptic and protein denaturation inhibitory assays. Results obtained shows that n-hexane fraction of P. dulcis seed stabilized stressed erythrocyte maximally (18.70 ± 3.23%) at 2.00 mg/mL and aqueous fraction (16.72 ± 1.97%) at 0.5 mg/mL, while aqueous extract highest % stability was 17.73± 2.32% at 2.00 mg/mL. Also, n-hexane fraction inhibited trypsin better at higher concentrations while the aqueous fraction was at a lower concentration. However, both showed better anti-tryptic activity than aqueous crude extract.Moreover, maximum percentage protein inhibitory activities for diclofenac (800 µg/ml), aqueous fraction (800 µg/ml), n-hexane fraction (1600 µg/ml) and aqueous extract (1600 µg/ml) were 72, 51, 35 and 32 respectively.This study concludes that almond seed fractions showed better anti-inflammatory activity compared to their crude extract. Therefore, the isolation of active compounds from these fractions may provide a novel anti-inflammatory drug.

DOI

10.21608/eajbsh.2022.265534

Keywords

Inflammation, Prunus dulcis, Membrane stabilization, Antitryptic, Protein denaturation

Authors

First Name

Omotosho

Last Name

Olatunde

MiddleName

Gbemiga

Affiliation

Department of Medical Biochemistry, Obafemi Awolowo University, Ile Ife, Osun State, Nigeria

Email

omotoshogo@oauife.edu.ng

City

Ile- Ife

Orcid

-

First Name

Oladiti

Last Name

Abidemi

MiddleName

Rashidat

Affiliation

Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile ife, Osun State, Nigeria.

Email

-

City

-

Orcid

-

First Name

Baruwa

Last Name

Solomon

MiddleName

Abayomi

Affiliation

Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile ife, Osun State, Nigeria.

Email

-

City

-

Orcid

-

First Name

Joseph

Last Name

Bawa

MiddleName

aphet

Affiliation

Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile ife, Osun State, Nigeria.

Email

-

City

-

Orcid

-

First Name

Oyelekan

Last Name

Ibukun

MiddleName

Esther

Affiliation

Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile ife, Osun State, Nigeria.

Email

-

City

-

Orcid

-

First Name

Idowu

Last Name

Oluwatimilehin

MiddleName

Iyanuoluwa

Affiliation

Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile ife, Osun State, Nigeria.

Email

-

City

-

Orcid

-

First Name

Akinpelu

Last Name

Ayinke

MiddleName

Bolajoko

Affiliation

Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile ife, Osun State, Nigeria.

Email

-

City

-

Orcid

-

Volume

13

Article Issue

2

Related Issue

35411

Issue Date

2022-12-01

Receive Date

2022-08-28

Publish Date

2022-10-18

Page Start

113

Page End

120

Print ISSN

2090-3812

Online ISSN

2090-3820

Link

https://eajbsh.journals.ekb.eg/article_265534.html

Detail API

https://eajbsh.journals.ekb.eg/service?article_code=265534

Order

10

Type

Original Article

Type Code

693

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, H. Botany

Publication Link

https://eajbsh.journals.ekb.eg/

MainTitle

Assessment of Anti-inflammatory Activity of Prunus dulcis [Miller D.A. Webb (ALMOND)] SEED Aqueous Extract and Fractions

Details

Type

Article

Created At

22 Jan 2023