The current study was carried out at the Faculty of Agriculture, Saba Basha, Alexandria University, and the horticultural research station, Sabahia, Alexandria, Egypt. From 2018 to 2022 assessment of genetic variations of Taxodium trees in Egypt based on the morphological description and DNA barcoding. Four different localities were designated to survey the Bald cypress (T. distichum) samples. The results recorded that the tree length ranged from 21.22 m (Giza tree) to 11.89 m (El-Beheria tree), while the Alexandria tree was recorded 17.30 m forward by Qalyubia tree in a range of 12.61 m. The tree trunk diameter (cm) ranged from 36.40 cm (El-Beheria tree) to 115 cm (Alexandria tree), while Qalyubia tree was recorded 46.40 cm forward by El-Beheria tree in the range of 36.40 cm. The tree condition for all the trees was very good except the tree from Giza governorate was moderate. From these data, we can describe the different Taxodium trees from different locations in Egypt to be genetic resources that can be used in the future in the breeding program. The data of T. distichum El-Beheria governorate using rbcL gene showed that the G+C content was 43.96% and the A+T content was 56.04%. in addition, the nucleotide number and molecular percentage were as follows:A (148) 27.92%;C (113) 21.32%; G (120) 22.64% andT (149) 28.11%; while T. distichumQalyubia Governorate, showed that the G+C content was 42.96% and the A+T content was 57.04%. in addition, the nucleotide number and molecular percentage were as follows A (148) 26.92%;C (113) 22.32%; G (120) 23.64% andT (149) 27.11%. The data of T. distichum_Alexandria Governorate showed that the G+C content was 43.96% and the A+T content was 56.04%. in addition, the nucleotide number and molecular percentage were as follows:A (148) 27.92%;C (113) 21.32%; G (120) 22.64% andT (150) 28.11%. finally, the data showed that the G+C content was 43.96% and the A+T content was 56.04%. in addition, the nucleotide number and molecular percentage were as follows:A (149) 27.92%;C (120) 21.32%; G (111) 22.64% andT (132) 28.11%. The data was recorded to the Protein in T. distichum_Giza Governorate using (rbcL) gene.