The current research was designed to evaluate the phytochemical contents and antimicrobial activity of Arthrocnemum macrostachyum (Moric) K. Koch, Suaeda pruinosa Lange and Kochia indica (Bassia indica) (Wight) A. J. Scott belongs to Chenopodiaceae (Amaranthaceae) family. This study revealed that in the case of A. macrostachyum the soil texture was clay loam, in the case of S. pruinosa the texture of the soil was sandy clay loam, while in K. indica the texture of the soil was loamy sand. S. pruinosa results showed the highest contents of total phenolics, flavonoids, lipids, carbohydrates and tannins in comparison with the remaining two plants, while A. macrostachyum showed the highesttotal alkaloids content. HPLC analyses resulted in, A. macrostachyum containingresorcinol, kaempferol and quercetin,in case of S. pruinosa contain ferulic acid, quercetin, kaempferol and resorcinol while, K. indica contain quercetin, kaempferol, resorcinol and phenantherine. Diethyl ether extract of S. pruinosa showed the highest antioxidant activity with scav % 95.25 followed by chloroform extract of A. macrostachyum (90.07%). The plants' extracts give moderate activity against Candida albicans, but in the case of Bacillus subtilis, S. pruinosa showed the highest activity. Also, K. indica results showed certain activity against Escherichia coli and Proteus Vulgaris