The aim of this study is a comparison between old cultivated commercial varieties (Giza 86, Giza 90 and Giza 88) and new varieties (Giza 94, Giza 95 and Giza 96) by evaluation of the fiber characteristics and the qualities of spinning. The fiber properties of the studied varieties on HVI and Nep tester instruments were made at Cotton Arbitration and Testing General Organization (CATGO) and measure the efficiency qualities of spinning yarn varieties for 3 yarn counts at Delta company (20 - 30- 40 'S for cotton varieties Giza 95 and Giza 90) and (50 - 60- 70 'S for Giza 94 and Giza 86) and (60 - 90- 120 'S for Giza 96 and Giza 88)).  The mean squares of cotton varieties for all studied fiber properties were highly significant differences as well as the two growing seasons mannered the same trend for all studied fiber properties as cotton varieties except fiber length, fiber elongation, short fiber index and neps count revealed insignificant differences.The interaction between six Egyptian cotton varieties and two growing seasons was recorded as highly significant for all studied fiber properties except for two traits fiber elongation and trash area. The yarn properties revealed high significance for the yarn traits of Giza 94 and Giza 86 for three yarn counts (50 - 60- 70 'S). The yarn properties (yarn strength(cN/tex) , thin places /400 m, thick places/400 m and yarn evenness (CV%)) under three yarn counts 20 ,30 and 40's of some Egyptian cotton varieties(Giza  95and Giza 90). Highly significant recorded for both of the Egyptian cotton varieties except yarn strength for both cotton varieties. The yarn properties were had recorded as highly significant for the yarn traits of Giza 96 and Giza 88 with regard to three yarn counts (60 - 90- 120 'S) except thick places/400m for cotton varieties. The interaction between Egyptian cotton varieties (Giza 94 and Giza 86) and yarn count is clear that highly significant for yarn properties. The interaction between Egyptian cotton varieties (Giza 95 and Giza 90) and yarn count had highly significant on yarn strength and thick places only.  The interaction between cotton varieties Giza 96 and Giza 88 and yarn count is highly significant for all yarn properties.