Bean yellow mosaic virus represents a stress effect on fava bean plants. This study was conducted to assess the lipid peroxidation and total phenolic content (TPC) of fava bean plants under bean yellow mosaic virus infection and its management by the biostimulants; Rhizobium and Bacillus species spraying application. The obtained data revealed that virus-infected plants maximized stress factors, the content of malondialdehyde (MDA), while it decreased in other applications. TPC significantly increased in Bacillus, and Rhizobium isolates spraying treatments with 20.99 and 22.44 mg/100g extract, respectively, compared with control. The current work provides bio-resistance by Bacillus or Rhizobium isolates effectively minimizes the adverse effects of challenges such as stressing viral infection and representing an environmentally friendly commercial value for plant growers.