The present study was carried out at Abess, Alexandria, Egypt, during the two seasons of 2018/2019 and 2019/2020 to study the performance of wheat varieties by different markers under drought. This experiment was conducted in a split-plot system in three replications during the two seasons. The main plots were irrigation treatments (Full irrigations (control), skipping one irrigation at the vegetative growth stage and skipping one irrigation at the heading stage), wheat varieties (Gemmieza 11, Giza 168, Giza171 and Shandaweel l) distributed in a subplot in both seasons. The obtained results showed that sowing the wheat cultivar Giza 171 or Giza 168 under normal irrigation recorded the highest mean value of yield, yield components, and protein (%) in grain under study conditions at Abess, Alexandria Governorate, Egypt.