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108312

Genetic Diversity of Egyptian Date Palms (Phoenix dactylifera L.) Using Morphological and Molecular Markers

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Last updated: 03 Jan 2025

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Abstract

Morphological and molecular analyses were conducted to evaluate the genetic diversity among eleven Egyptian date palms (Phoenix dactylifera L.). Ten SSR primer pairs revealed a high percentage of polymorphism (97.4%) among 38 total number of alleles, while 11 SCoT primers produced 48.5% from 171 amplified fragments. SCoTs recorded effective multiplex ratio (EMR) and PIC values of 7.5 and 0.14, respectively, opposing to 3.7 and 0.26 for SSRs, respectively. A similar marker index has thus resulted in both markers. Nei's gene diversity (h) was generally low for both marker systems indicating homogeneous nature and narrow genetic background for the studied Egyptian date palms, which was also emphasized through their high genetic similarity estimates. The results of UPGMA cluster analysis and PCoA grouped the eleven date palms according to their geographical location and sex. In addition to classical morphological characters, the current study confirmed the importance of using SCoTs as functionally gene-based multi-loci markers besides nuclear SSR loci to assess the genetic variations and cultivar identification among Egyptian date palm genotypes

DOI

10.21608/eajbsh.2019.108312

Keywords

Date palm (Phoenix dactylifera L.), SCoT, SSR, Morphological analysis, Genetic diversity, molecular markers

Authors

First Name

Sara

Last Name

Aly

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Ain Shams University, Cairo, Egypt

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City

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Orcid

-

First Name

Mohamed

Last Name

Saad

MiddleName

E.

Affiliation

Genomics Facility, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt. -Department of Biology, Faculty of Sciences, Taibah University, Al-Madinah Almonawarah, Kingdom of Saudi Arabia

Email

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City

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Orcid

-

First Name

E.

Last Name

Madboly

MiddleName

A.

Affiliation

The Central Laboratory for Date Palm Research and Development (CLDPRD), Agriculture\ Research Center (ARC), Giza, Egypt

Email

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City

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Orcid

-

First Name

Hoda

Last Name

Barakat

MiddleName

S.

Affiliation

Botany Department, Faculty of Science, Ain Shams University, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

H.

Last Name

El-Atroush

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Ain Shams University, Cairo, Egypt

Email

hala_elatroush@hotmail.com

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-

Orcid

-

Volume

10

Article Issue

2

Related Issue

8261

Issue Date

2019-12-01

Receive Date

2019-10-30

Publish Date

2019-12-01

Page Start

55

Page End

69

Print ISSN

2090-3812

Online ISSN

2090-3820

Link

https://eajbsh.journals.ekb.eg/article_108312.html

Detail API

https://eajbsh.journals.ekb.eg/service?article_code=108312

Order

6

Type

Original Article

Type Code

693

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, H. Botany

Publication Link

https://eajbsh.journals.ekb.eg/

MainTitle

Genetic Diversity of Egyptian Date Palms (Phoenix dactylifera L.) Using Morphological and Molecular Markers

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Type

Article

Created At

22 Jan 2023