Aflatoxins is one of the dangerous mycotoxins, especially aflatoxin B₁, where it is one of the causes of cancer disease to humans and animals, also leads to a large perdition. The current study was conducted on the investigation of environmental conditions in the local stores and their impact on contamination of aflatoxin B₁. Three models of economic crops: maize, wheat, and rice samples were collected from Baghdad and Wasit silos for six consecutive months. Sixty samples of the three grain crops were collected from the stores, (12) samples of cornand (24) samples each of wheat and rice, all samples with three replicates planted on the (PDA) medium. Then, Agricultural and ELISA tests were done. The results showed that the concentration of aflatoxin B₁ by ELISA technique in corn crop was more affected by the pollution compared with other crops. The concentration of aflatoxin B₁ in cornmeasured 46.2ppb, while rice crops scored less concentration of toxin reached to1.8 ppb.