In the current study, the levels of endogenous free and conjugated auxin (Indole-3-acetic acid, IAA), gibberellic acid (GA₃), and abscisic acid (ABA) were examined in two species of aquatic plants (Ceratophyllum demersum and Egeria densa). The comparison between the content of endogenous phytohormones in aquatic plants showed that C. demersumhad highestlevels of total (IAA, GA₃ and ABA) than E. densa. Different concentrations offree phytohormones extracts were prepared (0, 25, 50, and 100µl/l), then added to (MS) culture medium as an alternative to plant growth regulators. Effects of these concentrations on callus productionof black henbane (Hyoscyamusniger) and potato propagation (Solanum tuberosum) were studied in vitro. Freephytohormones extracts  with all concentrations encouraged production of callus tissue from leaves explant of black henbane compared with control treatment, also, these extracts promoted propagation of potatoby increasing number of nods and length of shoot.So, its appear that remnants of aquatic plants could be used successfully for agricultural improvement and another application of bioassay.