Two field experiments were carried out at Al- Sabhia, the Agricultural Research Station, Alexandria, Egypt during 2015/2016 and 2016/2017 seasons, to study the effect of mineral nitrogen with the bio-fertilizer (Rhizobacterin) and foliar application of boron levels on sugar beet.  The experiment was conducted a split-plot design in three replications, where the main plots were assigned for mineral- bio nitrogen fertilizer levels, while the subplots were occupied by two levels of boron. The results indicated that increasing nitrogen fertilizer levels from 60 up to 100 kg N/fed combined with bio- fertilizer (Rhizobacterin) significantly increased fresh top yield, sugar yield, T.S.S.%, sucrose percentage and purity of sugar beet through the two seasons. Root yield significantly increased by increasing boron fertilizer levels from 0.5 up to 1 kg B /fed Application of mineral with bio- nitrogen fertilizers and foliar application of boron were associated with the significant effect on crop growth rate, leaf area index, root yield, sugar yield, TSS% and sucrose% under study conditions.