The present study aimed to evaluate the biochemical effect of COVID-19 vaccines (Pfizer‐BioNTech COVID‐19 vaccine). 90 samples were included in the study from people in the vaccine center in Al-Dhuluiya general hospital-Salah Al-Din who was vaccinated with the Pfizer vaccine during the period from 1/11/2021 until 1/2/2022. The samples were collected before receiving the 1st dose and after 24-48 hours of taking the 1st and 2nd doses. The collected samples were divided into three groups: -First group-G1: Before taking the first dose of the Pfizer vaccine, -Second group-G2, and Third group-G3: After taking the first and second doses of the Pfizer vaccine respectively. The study includes the determination of different biochemical parameters such as serum Troponin, Lactate dehydrogenase-LDH, D-dimer, Lipid profiles (Cholesterol, Triglycerides, High-density lipoprotein cholesterol-HDL-C, Low-density lipoprotein cholesterol-LDL-C), Total iron, Transferrin, ferritin, Glutathione-GSH, Malondialdhyde-MDA, and peroxy nitrate-PON.




The results showed that LDH, D-dimer, and MDA levels were significantly elevated after the first and second doses of the Pfizer vaccine as compared with their levels before receiving doses vaccine. While the level of troponin, lipid profile, total iron, and transferrin didn't show any significant difference after vaccination as compared with their levels before receiving the vaccine. So, we can conclude that the vaccination with the Pfizer‐BioNTech COVID‐19 vaccine may affect the cardiac marker and the oxidative stress state.