The primary reason behind the failure/setback of significant antibiotics is the bacterial resistance to antibiotics and their entry into the cell. Incorporating metallic nanoparticles with antibacterial characteristics, nanotechnology provides a dynamic strategy for creating novel formulations. Therefore, the goal of this study was to design the silver nanoparticles (AgNPs) as the first step in our study rationale, and by then the second step will be used as an efficient alternative treatment to obliterate the multi-resistant bacteria. The synthesized materials were characterized using X-ray powder diffraction (XRD) and transmission electron microscope (TEM). The XRD studies revealed that the synthesized AgNPs have face-centered cubic. TEM showed AgNPs with spherical regular smooth shapes. AgNPs have a zeta average of 152.9 nm as confirmed also by TEM. This study could be postulated that the higher NPs concentrations significantly increase antibacterial efficacy. AgNPs, however, can be investigated as a significant opportunity for more study since they have a lot of potential as an antibacterial agent, according to the verdicts of the current work.