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Bioinformatics Analyses of The Complete DNA Genome of An Egyptian Isolate of Banana bunchy Top Virus

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Last updated: 03 Jan 2025

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Abstract

Banana bunchy top disease (BBTD) caused by Banana bunchy top virus (BBTV) was recorded in the early twentieth century in Egypt. It is considered the most economic impact on banana yield productivity.Bioinformatics analyses of the complete genome of an Egyptian isolate of BBTV compared to overseas BBTV isolates or strains and other Nanoviruses were aimed. Banana leaf samples naturally infected with BBTD were collected from the open field and the presence of BBTV was detected via PCR. Bioinformatics analyzes of the BBTV-DNA genome were also studied. The experimental results showed that BBTV isolates of this study had a genome that consists of six encapsidated single-stranded DNA components (BBTV-DNA-R (LC468138, -U3 (LC468139), -S (LC468140), -M (LC468141), - C (LC468142) and –N (LC468143)) of ~ 1.1 kb in length, each with one open reading frame (ORF) in the virion-sense Two conserved regions (Common region-stem loop (CR-SL) and common region-M (RC-M)). TATA box, i.e., non-nucleotide potential TATA and polyadenylation signals adjacent to GC-rich regions that contain the TTG sequence were found. Differences ranging from 0.55 to 4.60% were recorded when BBTV components were compared to the most similar oversea BBTV strains of seven countries. Phylogenetic trees confirmed the genetic relationships among the high percentage of similarity between the compared BBTV strains. Families of Gene domains, restriction enzyme maps and differences between BBTV components and those of nano viruses were also discussed. The six BBTV-DNA components contain ORFs encoding the genes of rolling-circle replication initiation protein (rp < /em>, DNA-R), a protein of unknown function (DNA-U3), a coat protein peptide (cp < /em>, DNA-S), movement protein (mv, DNA-M), cell cycle link protein (ccl, DNA-C) and a nuclear shuttle protein (ns, DNA-N), respectively. Further studies should be done using molecular docking tools in a trial to find a suitable control strategy for BBTV.

DOI

10.21608/eajbsg.2022.248724

Keywords

BBTD, BBTV, Bioinformatics analyzes, genome, domains, Nanoviruses

Authors

First Name

Abdel Razek

Last Name

S.

MiddleName

Fatma

Affiliation

Department of Agricultural Microbiology (Laboratory of Virology), Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shobra, Cairo, Egypt

Email

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City

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Orcid

-

First Name

El-Masry

Last Name

S.

MiddleName

Samar

Affiliation

Department of Agricultural Microbiology (Laboratory of Virology), Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shobra, Cairo, Egypt

Email

samar_sayed@agr.asu.edu.eg

City

-

Orcid

-

First Name

Ibrahim

Last Name

D.

MiddleName

S.

Affiliation

Agricultural Genetic Engineering Research Institute, Agricultural Research Center, 9 Gamaa st., P.O. Box, 12619, Giza, Egypt

Email

-

City

-

Orcid

-

First Name

El-Arabi

Last Name

F.

MiddleName

T.

Affiliation

Department of Agricultural Microbiology (Laboratory of Virology), Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shobra, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Mahmoud

Last Name

Khalifa

MiddleName

E.

Affiliation

Botany and Microbiology Department, Faculty of Science, Damietta University, Damietta, Egypt

Email

-

City

-

Orcid

-

First Name

Sadik

Last Name

S.

MiddleName

A.

Affiliation

Department of Agricultural Microbiology (Laboratory of Virology), Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shobra, Cairo, Egypt

Email

-

City

-

Orcid

-

Volume

14

Article Issue

2

Related Issue

35532

Issue Date

2022-12-01

Receive Date

2022-05-02

Publish Date

2022-07-08

Page Start

1

Page End

18

Print ISSN

2090-0872

Online ISSN

2090-0880

Article File

EAJBSG_Volume 14_Issue 2_Pages 1-18.pdf

PDF . 1.2MB

Link

https://eajbsg.journals.ekb.eg/article_248724.html

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https://eajbsg.journals.ekb.eg/service?article_code=248724

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Original Article

Type Code

689

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, G. Microbiology

Publication Link

https://eajbsg.journals.ekb.eg/

MainTitle

Bioinformatics Analyses of The Complete DNA Genome of An Egyptian Isolate of Banana bunchy Top Virus

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Article

Created At

22 Jan 2023