Background: Typhoid is one of the infectious diseases, slide and tube agglutination will remain not relevant as a diagnosis tool for typhoid fever in the poor countries, slide and tube agglutination have poor sensitivity and specificity, the results should be confirmed by a new diagnostic method. Objective: This study aimed to find a new diagnostic method that is more sensitive to typhoid fever. Therefore, micro-titer plate assays are inexpensive and do not require special equipment. Material and methods: In the current study, the blood samples were collected from 1000 clinically suspected cases of typhoid fever attending outpatients and inpatients clinic of "Faquos Fever Hospital" from Dec. 2016 to Dec. 2019, and laboratory investigations were blood culture, micro-titer plate, slide and tube agglutination test. Results: Among (1000) clinically suspected cases. Regarding sex 56% were male, and females (44%). Clinically suspected fever cases with fever 5 days, the age of the studied cases ranged from 8 to 94 years with a mean of 35.55± 19.65 years, and there is no infection by paratyphoid A and Conclusion: Diagnosis of salmonella antibodies by micro-titer plate wells are reliable, this method is simple, highly sensitive, specific, and quantitative in diagnosing typhoid fever when compared with different diagnostic method.