Newcastle virus is one of the risk factors of the chicken industry in Egypt and the world. The affected chickens have a significant death rate.   The current study's major goals are to isolate and characterise Newcastle disease virus isolates, as well as to optimise chicken-associated feeding with probiotics.The sample of white leghorn Chicken organs was done from diseased Chickens collected from different commercial Chickens farms of Qaluybia, n=25,  El Sharkia, n=20 El Gharbia , n=15 and El Monofia, n=15 governorates depending on distinct NDV like viral symptoms from January to May 2019 and 2020. NDV isolates were isolated and identified using non-NDV vaccinated chicken eggs that were 9-11 days old. The findings show that both velogenic neurotropic and velogenic viscerotropic strains of NDV are common. In hens utilising hemagglutination inhibition, seroprevalence shows strong antibody titers against NDV (HI).  The probiotics as adaptive to feed healthy and NDV velogenic infected and chickens due to improving the immunity by increasing the cells immunity of differential leukocyte blood cell profile, Eosinophil, Lymphocyte, Monocyte cell count and Blood protein serum values, (Total Protein, Albumin and Globulin). While differential leukocyte blood cell profile and Blood protein serum values were decreased in NDV velogenic infected chickens compared to standard blood. Except total neutrophils were increased in infected chickens blood compared decreased in Healthy and infected chickens' blood with Standard blood. In conclusion, in Egypt, a number of velogenic NDV strains are currently circulating, and the inclusion of probiotics in chicken feed improves their resistance to NVD infection.