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134701

Occurrence and Control of Alternaria alternata, Penicilliun citrinum and Aspergillus flavus Mycotoxins in Broad Bean Seeds by Benzoic and Sorbic Acids

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Last updated: 22 Jan 2023

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Abstract

Infected broad bean seeds were collected from Noubaria region during the season 2017-2018. Fungi isolated and purified from collected seeds were identified as Aspergillus flavus, Penicilliun citrinum and Alternaria alternata. The occurrence frequencies of the isolated fungi were 9.08, 15.16 and 75.76 %, respectively. Production potentials oftoxin by Alternaria alternata and P. citrinum isolates, as estimated by the Plug Agar method, were 23100 and 33700 ng/g, respectively. Production of aflatoxins by A. flavus isolates not detected. Sorbic and benzoic acids were showed to be more effective in reducing the growth of A. alternata and P. citrinum than metalaxyl and ridomyl fungicides. A. alternata was less sensitive to sorbic acid than benzoic acid. All the tested treatments significantly reduced the growth of A. alternata growth and alternariol (AOH) production; however, a higher efficiency ratio (99.86%) was realized by benzoic acid. Similar inhibition of growth and citrinin (CTN) production was found on P. citrinum. Metalaxyl was less efficient in inhibiting AOH and CTN toxins than the other tested treatments. The efficiency of benzoic and sorbic acids to reduce AOH and CTN production was higher by soaking seed treatment.  Seeds inoculated with the tested fungi, then treated with sorbic or benzoic acid found to be healthy with normal color, texture and morphology.

DOI

10.21608/eajbsg.2020.134701

Keywords

Mycotoxins, broad bean, benzoic acid, sorbic acid

Authors

First Name

Ahmed

Last Name

Heflish

MiddleName

I. A.

Affiliation

Department of Agricultural Botany, Faculty of Agriculture (Saba-Basha), Alexandria University, Alexandria, Egypt

Email

ahmed_heflish@yahoo.com

City

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Orcid

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First Name

I.

Last Name

El samra

MiddleName

A.

Affiliation

Department of Agricultural Botany, Faculty of Agriculture (Saba-Basha), Alexandria University, Alexandria, Egypt

Email

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City

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Orcid

-

First Name

N.

Last Name

Youssef

MiddleName

H.

Affiliation

Regional Centre for Food and Feed, Alexandria, Agricultural Researches Centre, Egyptian Ministry of Agriculture, El -Giza, Cairo, Egypt

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Volume

12

Article Issue

2

Related Issue

16038

Issue Date

2020-12-01

Receive Date

2020-10-21

Publish Date

2020-12-01

Page Start

75

Page End

87

Print ISSN

2090-0872

Online ISSN

2090-0880

Link

https://eajbsg.journals.ekb.eg/article_134701.html

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https://eajbsg.journals.ekb.eg/service?article_code=134701

Order

8

Type

Original Article

Type Code

689

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, G. Microbiology

Publication Link

https://eajbsg.journals.ekb.eg/

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Article

Created At

22 Jan 2023