Arthrospira platensis considered a storehouse rich in nutritionally active compounds with dietary and pharmaceutical applications. Gamma-Linolenic acid (GLA) or ώ-6, which characterized by several therapeutic properties, can be obtained safely from A. platensis. This study aimed to stimulate GLA productivity by A. platensis, through using different culturing conditions of temperature and light regime as well as hydrogen peroxide. The obtained results reflected that continuous lighting regime (24L) enhances the production of the unsaturated fatty acid especially GLA than the regime of 12 hours darkness: 12 hours lighting (12D:12L), at the same time low temperature (24^oC) enhance GLA productivity (29.98 mg. g^-1 dry weight). Although hydrogen peroxide (H₂O₂) did not support biomass production, its low concentrations induced the GLA productivity. The cited results clarified that the maximum GLA (31.62 mg. g^-1) was obtained under continuous lighting regime, at 24^oC by adding 2 mM H₂O₂, forming 27.42% from the total fatty acids. Nevertheless, with the increase in population accompanied by increasing their needs, researchers will compete to obtain the maximum productivity of A. platensis for GLA with its numerous therapeutic properties.