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16711

Sensitivity of nested PCR toward human cytomegalovirus-DNA in native sera and in extracted DNA of serum samples

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Last updated: 22 Jan 2023

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Abstract

            PCR has been commonly used for genomic viral diagnosis for its sensitivity and accuracy. It showed a higher sensitivity when compared to virus isolation in tissue culture and also in antigenemia detection. Definitely, DNA samples are critical factor in PCR validity. Out of 84 serum samples subjected to this study and extracted by Wizard® DNA purification mini kit, 27 samples (32.1%) were positive PCR. While, 15 samples (17.9%) only out of the same population study (84) were positive PCR for HCMV DNA in native serum samples (without DNA extraction). This result was confirmed the importance of DNA extraction from serum samples for detection of HCMV which, subsequently lead to more sensitive diagnostic tool of an ongoing HCMV infection.

DOI

10.21608/eajbsg.2010.16711

Keywords

HCMV DNA, HCV RNA, nested PCR, DNA extraction, serum

Authors

First Name

Sahar

Last Name

Shoman

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Affiliation

Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt

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Orcid

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First Name

Ashraf

Last Name

Tabl

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Affiliation

Department of Biomedical Technology, National Research Center, Giza, Egypt

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Orcid

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First Name

Hussam

Last Name

Ghanem

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt

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Orcid

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First Name

Mohamed

Last Name

Nabil

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-

Affiliation

Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt

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Volume

2

Article Issue

1

Related Issue

3544

Issue Date

2010-06-01

Receive Date

2018-10-15

Publish Date

2010-06-01

Page Start

1

Page End

8

Print ISSN

2090-0872

Online ISSN

2090-0880

Article File

EAJBSG_Volume 2_Issue 1_Pages 1-8.pdf

PDF . 465.3kB

Link

https://eajbsg.journals.ekb.eg/article_16711.html

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https://eajbsg.journals.ekb.eg/service?article_code=16711

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Original Article

Type Code

689

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Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, G. Microbiology

Publication Link

https://eajbsg.journals.ekb.eg/

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Article

Created At

22 Jan 2023