This study investigated knowledge about HIV/AIDS and tuberculosis coinfections among students of tertiary Institutions in Ilorin, Nigeria. A multistage sampling technique was adopted to select 405 students from the three selected tertiary Institutions (i.e. University of Ilorin, Kwara State Polytechnic and Kwara State College of Education, Ilorin). A cross-sectional survey research design was carried out, using a structured questionnaire, which was tested for reliability at 0.89r. The data gathered were analysed by descriptive statistics of frequency count and percentage to analyse the bio-data and research questions, while inferential statistics of Analysis of Variance (ANOVA) was employed to analyse the hypotheses at 0.05 alpha level. Findings revealed that, significant differences existed between the students of tertiary Institutions in Ilorin in their knowledge about reciprocal interaction and ease of detection of HIV/AIDS and tuberculosis co-infections. Whereas, not significant difference in their knowledge about HIV/AIDS and tuberculosis co-infections being treatable. It was recommended that the school management should incorporate health information, education and communication in the curriculum regardless of the course the students is studying, they should also make available the health education and information leaflets to the students