Regular environmental monitoring samples for microbiological quality of different locations in pharmaceutical facility are by far very critical measure in controlling clean area and environment which have direct impact on the microbiological quality of the final pharmaceutical products. Different methods were used to collect samples from different locations. These samples were processed and followed by isolation and identification. The obtained data were analyzed to find microbial distribution in the environment of pharmaceutical facility. Twenty one bacterial and fungal species were isolated from different locations in the pharmaceutical facility. Group of genus Staphylococci contributes to 38.4% of total positive samples while genus Micrococci contributes to 22.4% and Gemella morbillorum represents 1.1%. Genus Bacilli represents 35.0%, Candida albicans 1.3%, Klebsiella pneumonia 1.0% and Stenotrophomonas maltophilia 0.01%. Most microorganisms found among 7 identified genuses were belonging to Staphylococcus, Micrococcus and Bacillus species.