Beta
ManageSearchChatDiscover
16657

A Potential 17- β Estradiol degrader Bacterium Isolated from Sewage water

Article

Last updated: 22 Jan 2023

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

A successful 17 β estradiol (E2) degrading bacterium was isolated from waste water of Saft Elhena drain in Egypt.  Molecular identification of the isolated bacteria was carried out using DNA (RAPD), the amplification was made by restriction analysis of amplified 16s rDNA and sequencing. Alos, the bacterium was incubated in saline media under different levels of 17 β-estradiol anaerobically. Levels of CO2 production were examined by GC and residual 17 β-estradiol was tested by HPLC to measure biodegradation capacity. Identification of the genetic element responsible of the gene resistance in the bacteria was tested. The sequence analysis of 16S rRNA gene for 17 β estradiol degrader bacterium showed that its affiliations to phylum Enterobacteriaceae, and  it belonged to the genera Klebsiella sp. Results revealed that Klebsilla was cabable of degrading estradiol and that the responsible resistance gene was mostly the chromosomal gene. However, further studies are still going on to locate and examine the gene responsible for E2 resistance.

DOI

10.21608/eajbsg.2012.16657

Keywords

17 β-estradiol, Degradation, Curing, carbon production

Authors

First Name

Nagwa

Last Name

Elnwishy

MiddleName

-

Affiliation

-Department of Biotechnology, center of chemistry and chemical engineering, Lund university, Sweden -Biotechnology Research Center (BRC), Suez Canal University, (New Campus), Ismailia 41522, Egypt

Email

-

City

-

Orcid

-

First Name

Amro

Last Name

Hanora

MiddleName

-

Affiliation

-Biotechnology Research Center (BRC), Suez Canal University, (New Campus), Ismailia 41522, Egypt -Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt

Email

-

City

-

Orcid

-

First Name

Raafat

Last Name

Afifi

MiddleName

-

Affiliation

-Department of Marine Biology, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt -Department of Biology, Almadinah Almonawara, Tybah university, Kingdom of Saudi Arabia

Email

-

City

-

Orcid

-

First Name

Helmy

Last Name

Omranf

MiddleName

-

Affiliation

-Department of Marine Biology, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt -Department of Food Technology, faculty of Agriculture, Suez Canal University, Ismailia 41522,, Egypt

Email

-

City

-

Orcid

-

First Name

Bo

Last Name

Matiasson

MiddleName

-

Affiliation

Biotechnology Research Center (BRC), Suez Canal University, (New Campus), Ismailia 41522, Egypt

Email

-

City

-

Orcid

-

Volume

4

Article Issue

1

Related Issue

3536

Issue Date

2012-12-01

Receive Date

2018-10-14

Publish Date

2012-12-01

Page Start

27

Page End

34

Print ISSN

2090-0872

Online ISSN

2090-0880

Article File

EAJBSG_Volume 4_Issue 1_Pages 27-34.pdf

PDF . 314.0kB

Link

https://eajbsg.journals.ekb.eg/article_16657.html

Detail API

https://eajbsg.journals.ekb.eg/service?article_code=16657

Order

4

Type

Original Article

Type Code

689

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, G. Microbiology

Publication Link

https://eajbsg.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023