Background: Urinary tract infection (UTI) is one of the health problems in the community in all ages and both sexes. Many organisms are involved  particularly enteric bacteria in females. Antimicrobial resistance of bacterial pathogen are complaining treatment and recovery of infected individuals. So UTI become more complicated and  recurrent trouble maker infection for community. Aims of the study This study was devoted to focus on the main bacterial pathogens causing UTI in adult patients from both sexes in Hit District as well as to show the Antibiogram of the predominant bacterial isolate from patients. Patients and methods Mid- stream urine specimens were collected from adult males and females, each specimen was cultivated on blood agar and Mac Conkey agar and incubated at 37C aerobically for 24 hours. Bacterial isolates were identified with Gram stain and biochemical tests. Predominant bacterial isolate, E. coli was tested for antimicrobial sensitivity test by disk diffusion test (Kerby-Bauer Technique). Results Adults females were showing higher rate of UTI than adult males (77.8 %), (263) and (59.6%), (37) respectively. E. coli took first rank of isolation in both sexes, 26.5 % in males and 40.8% in females. Staphylococcus became next (73),              (25.26%) while proteus mirabilis was showing third rank of isolation in both sexes.  Regarding Antibiogram, E coli showed high sensitivity for imipenium (95.5%) followed by Amikacin (90.2%) while studied E coli isolate showed high tare of resistance to Ampicillin and Cotrimasol-Methprim. Conclusion: We can conclude from this study that UTI is an important infection in community and enteric bacteria particularly E. coli  is an important causative agent. So precautions must be taken by people to prevent infection. Community education  in self  hygiene  and  social status must be done.  Physicians must care of  multi drug resistant E. coli during manipulation UTI