Three hundred and thirty two (n=332) urine specimens were collected from patients attending different hospitals in  KhartoumState during the period from May to November 2011. The isolates were transported to the microbiology lab (research lab) at SudanUniversity for Sciences and Technology. Identification of isolates was done by using conventional biochemical methods and microbact 2000 24E (OXOID) system. All Isolates were then stored at –70°C in Tryptic Soy broth with 20% glycerol. The isolated bacteria were tested for their antimicrobial resistance.  ESBL screening was confirmed bydouble disk synergy test. The results showed that urinary Gram negative bacteria was 65.7%. High rate was caused by E. coli (72.0%) followed by K. pneumoniae (14.7%). Maximum sensitivity was seen for imipenem (100%), followed by piperacillin/tazobactum (91.7%) and cefoxitin (87.2%). The maximum resistance was seen against ceftazidime (74.8%) followed by cefotaxime (70.6%). ESBL producing bacteria was (59.6%) mostly were in K. pneumoniae (68.8.%) followed by E. coli (65.0 %). ESBL producing bacteriashowed maximum resistance to ceftazidime (95.4%), followed by cefotaxime (94.6%), while minimum resistance was seen with imipenem (0%), followed by piperacillin/tazobactam (3.8%) and cefepime (7.7%).