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16631

Characterization and Identification of Moderately Thermophilic Bacteria Isolated from Jazan Hot Springs in Saudi Arabia

Article

Last updated: 22 Jan 2023

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Abstract

Three
new moderately thermophilic bacteriawere isolated from three (
Al-Khawbah, Bani Malik, and
Al-Bozah
)
hot springs located in Jazan district, south-western of Saudi Arabia.The isolates
were identified by the sequence analysis of the 16S rRNA gene. The 16S rRNA
gene sequence alignment with GenBank resulted in a percent identity (% ID) with
closest GenBank match of 98% suggesting that the isolates are new strains
within the branching of well-defined genus Bacillus. The phylogenetic analysis
of these strains using their 16S rDNA sequence data showed that strains SA and
SS30 had highest homology (98%) with B. licheniformis, whereas strain
SA09 showed 98% similarity with B. subtilis. The morphological,
biochemical, and physiological characteristics
of the isolates were also studied. They were aerobic, gram-positive, rod-shaped,
and moderately thermophilic bacteria (with an optimum growth temperature of
45–50˚C, and pH of 6).

DOI

10.21608/eajbsg.2014.16631

Keywords

Thermophilic bacteria, Bacillus, Hot spring, isolation, 16s rRNA gene

Authors

First Name

Mohammed

Last Name

Sarhan

MiddleName

A. A.

Affiliation

Department of Biological Sciences, College of Science, King Khalid University, Saudi Arabia

Email

mohammed_sarhan@yahoo.com

City

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Orcid

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First Name

Saad

Last Name

Alamrri

MiddleName

-

Affiliation

Department of Biological Sciences, College of Science, King Khalid University, Saudi Arabia

Email

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City

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Volume

6

Article Issue

1

Related Issue

3531

Issue Date

2014-06-01

Receive Date

2018-10-14

Publish Date

2014-06-01

Page Start

67

Page End

75

Print ISSN

2090-0872

Online ISSN

2090-0880

Link

https://eajbsg.journals.ekb.eg/article_16631.html

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https://eajbsg.journals.ekb.eg/service?article_code=16631

Order

7

Type

Original Article

Type Code

689

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, G. Microbiology

Publication Link

https://eajbsg.journals.ekb.eg/

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Article

Created At

22 Jan 2023