Sixty two (62) samples were collected from out and inpatients admitted to Tikrit teaching hospital in Tikrit city. these specimens were collected from sputum of immunocopromised patients  of both sexes and different ages. 19(30.6%) isolates from patients sputum, were infections more A. fumigatus (12.9%), A. terreus (6.5%), and P. sp (3.3%) while were (1.6%) of A. niger, A. flavus, F. solani, P. chrysogenum and A. alternata. The isolated were identified according morphological and Cultural characteristic.  It emerges from the study that fungal isolates produced various virulence factors since, hemolysin, protease and phospolypase production were produced in A. fumigatus (62.5%) hemolysin and phospholipase production, and  (87.5%) protease production, while A. terreus protease and phospholypase production were  (50%) and (25%) of hemolysin production, while A. flavus, P. chrysogenum and A. alternata isolates were no produced protease. P. chrysogenum were produce only hemolysin (100%). All isolates of A. niger, F. solani and A. alternata were phospolypase production (100%).