The comparative study of the effect of locally made black soap and some medicated soaps like Dettol, Delta, Tetmosol, and Septol were investigated on isolated human skin bacteria using disk diffusion method. The bacteria isolated from the skin were Bacillus species, Klebsiella pneumoniae, Staphylococcus aureus, and Staphylococcus species. The results obtained revealed that Tetmosol soap exhibited the highest level of antibacterial activity, which result in the greatest zones of inhibition than other soaps; Black soap, Dettol, Delta, Tetmosol, and Septol on the isolated human skin microflora. Tetmosol soap through this study can thus, be recommended for use since it has the potentials of treating skin diseases caused by these isolated microflora.