This study was intended to isolate and  identification of Candida albicans which causing vaginitis in women. Atotal 204 samples of vaginal tract swabs were collected from patients women from April to November, 2015 in Dyala/Iraq. Standard microbiological methods were used to isolate and identify candida from vaginal swabs obtained from study object, sabouroud dextrose agar used for isolation of candida, CHROMagar of candida used to identification of C. albicans, germ tube test done. The final identification done by using VITEK Yeast Biochemical Card. From 204 infected women 75 have vaginal candidiasis about (36.76%), C. albicans was causative agent of 54 isolates about (72%). The genetic diversity of recent clinical isolates of Candida albicans was studied basis on amplified DNA bands fragment that spans the side of the transposable intron in the 25s rDNA by using CA-INT-L and CA-INT-R primers. Our analysis of 54 C. albicans isolates showed that genotype-A with (450bp)band presented in 27 isolates about (50%) was a predominant C. albicans genotype, genotype-B with (840bp)band was found in16 isolates with (29.62) ratio, and finally genotype-C with both bands (450-840bp), was found in 11 isolates about (20.37%).