Petroleum oil is the
main pollutant specially in petroleum countries producers. In This research study
looked for bacterial strains have ability to degrade petroleum oily pollutants.
Two bacterial isolates from 23 isolates have high ability to degrade engine oil were
characterized and identified. The two bacterial isolates have been identified
phenotypically and genotypically methods, as Bacillus subtilis strain
BS1 and Bacillus axarquiensis strain BA1, these deposited in national
center for biotechnology information (NCBI Gen Bank) under the Accession
numbers MF375213.1 and MF375110.1 respectively. Two isolated strains have
ability to grow in minimal medium containing petroleum oil with maximum
bacterial growth was determined by spectrophotometry. Bacillus subtilis growth exhibited
1.7 OD at 600nm after 2 days of incubation time whereas Bacillus axarquiensis
exhibited 1.14 OD at 600 nm after 3 days of incubation time with minimal
medium containing 2% petroleum oil.