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16314

Purification and Characterization of Fibrinolytic Enzyme Produced by Bacillus subtilis Egy.

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Last updated: 22 Jan 2023

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Abstract

A novel extracellular enzyme with strong fibrinolytic activity, produced by Bacillus subtilis Egy, which was isolated from the Egyptian soil was purified and characterized. The enzyme was secreted by cultured B. subtilis Egy under solid state fermentation and purified 21.5 purification fold using precipitation by ammonium sulphate followed by ion exchange chromatography, and gel filtration on Sephadex G- 100 chromatography. Purified enzyme showed optimum activity at 50oCreaction temperature and pH 8. Enzyme stability studies revealed that the enzymeis stable up to 30 °C and it retained 80% of its activity at 40oC.In addition, the purified enzyme is stable at pH 8-9. The fibrinolytic activity was enhanced by Mn2+,Cu2+, Ca2+, Na+,  and Ba2+ and inhibited by, Zn2+, and Hg2+. Moreover, the activity was decreased by EDTA and PMSF at 2 mM final  concentration.

DOI

10.21608/eajbsg.2018.16314

Keywords

Bacillus subtilis Egy, Fibrinolytic enzyme

Authors

First Name

Nora

Last Name

Elgamal

MiddleName

N.

Affiliation

Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Egypt

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First Name

Fawkia

Last Name

El- Beih

MiddleName

-

Affiliation

Faculty of Science, Ain Shams University, Cairo, Egypt

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City

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Orcid

-

First Name

Saadia

Last Name

Hassnin

MiddleName

M.

Affiliation

Faculty of Science, Ain Shams University, Cairo, Egypt

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Orcid

-

First Name

Maysa

Last Name

Moharam

MiddleName

E.

Affiliation

Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Egypt

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-

City

-

Orcid

-

First Name

Magda

Last Name

El-Bendary

MiddleName

A.

Affiliation

Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Egypt

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City

-

Orcid

-

First Name

Mostafa

Last Name

Abo Elsoud

MiddleName

M.

Affiliation

Microbial Biotechnology Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Egypt

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Orcid

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Volume

10

Article Issue

1

Related Issue

3490

Issue Date

2018-06-01

Receive Date

2018-10-11

Publish Date

2018-06-01

Page Start

59

Page End

68

Print ISSN

2090-0872

Online ISSN

2090-0880

Article File

EAJBSG_Volume 10_Issue 1_Pages 59-68.pdf

PDF . 526.8kB

Link

https://eajbsg.journals.ekb.eg/article_16314.html

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https://eajbsg.journals.ekb.eg/service?article_code=16314

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Original Article

Type Code

689

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, G. Microbiology

Publication Link

https://eajbsg.journals.ekb.eg/

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Article

Created At

22 Jan 2023