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16313

Identification of Endoglucanase Gene Responsible for Cellulose Degradation Using Aspergillus flavus

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Last updated: 22 Jan 2023

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Abstract

Twenty
two of cellulose-degrading fungal isolates were isolated from five different
samples; rotted sugar cane bagasse, rotted plant, termites, soil and animal
manure. Out of 22 isolates, 21 strains showed hydrolyzing zone on agar plates
containing carboxy methyl cellulose after iodine staining. The fungal isolate
No. S4, exhibited the highest endoglucanase (CMCase) activity with (0.165
IU/ml) in cellulase production culture. The best
exoglucanase (FPase) and endoglucanase (CMCase) of fungal isolate S4 was
obtained after incubation at 30°C for 7 days. Sugar cane bagasse (SCB) induced
the production of FPase and CMCase with maximum activity of 3.6 fold and 2.2
fold, respectively more than that of the maximum yield in case of
carboxy methyl cellulose (CMC). The fungal isolate S4 was identified as Aspergillus
flavus on the basis of 18S rRNA and ITS region sequence analysis.

DOI

10.21608/eajbsg.2018.16313

Keywords

Aspergillus flavus, cellulose degradation

Authors

First Name

Mustafa

Last Name

El-Bakary

MiddleName

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Affiliation

Agricultural Genetic Engineering Research Institute, Agricultural Research Center, Giza, Egypt

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Orcid

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First Name

Khaled

Last Name

ElBaghdady

MiddleName

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Affiliation

Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt

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Orcid

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First Name

Amr

Last Name

Ageez

MiddleName

-

Affiliation

Faculty of Biotechnology, October University for Modern Sciences and Arts (MSA), Egypt

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Volume

10

Article Issue

1

Related Issue

3490

Issue Date

2018-06-01

Receive Date

2018-10-11

Publish Date

2018-06-01

Page Start

47

Page End

57

Print ISSN

2090-0872

Online ISSN

2090-0880

Article File

EAJBSG_Volume 10_Issue 1_Pages 47-57.pdf

PDF . 622.0kB

Link

https://eajbsg.journals.ekb.eg/article_16313.html

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https://eajbsg.journals.ekb.eg/service?article_code=16313

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Original Article

Type Code

689

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, G. Microbiology

Publication Link

https://eajbsg.journals.ekb.eg/

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Article

Created At

22 Jan 2023