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272456

Morphological and Molecular Characterization Based on ITS-2 of Moniezia expansa Rudolphi, 1810 (Anoplocephalidae) Isolated from The Intestine of Sheep, Ovis aries (Bovidae) from E

Article

Last updated: 03 Jan 2025

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Abstract

One hundred and twenty small intestines of sheep obtained from the Basateen Automated Slaughterhouse in Cairo, Egypt, between February 2021 and May 2021 were examined for cestodes. The cestode observed was Moniezia expansa (10.8%). It was measured 175-200 cm in length and varied in width according to the maturation of proglottids. Scolex was measured 0.75-0.88 mm in size and composed of a clear apical region without a rostellum. It had four conspicuous suckers with diameters of 0.25-0.31 mm. Scolex was followed by an unsegmented neck and proglottids. Proglottids were substantially wider than they were long. The immature proglottids measured 0.35-0.60 mm in length and 1.97-3.20 mm in width, without obvious structures. While mature proglottids were measured 0.70-1.40 mm in length and 6.75-8.25 mm in width and possess completely formed reproductive organs. The gravid proglottids were measured 1.38-4.43 mm in length and 14.25-16.75 mm in width, packed with eggs, and came towards the end of the strobila. PCR amplification produced a fragment of approximately 800 bp in size. The analyses showed distinct genotypes with sequence identities that ranged from 98.90% to 100% when compared to the GenBank sequences of M. expansa. The variation of evolutionary divergences was extended from 0.000 to 1.356 for Moniezia spp. and another genus of cestodes. Based on morphological traits and molecular analyses, the currently isolated worms were confirmed as M. expansa.

DOI

10.21608/eajbse.2022.272456

Keywords

Cestodes, Moniezia expansa, Scolex, Proglottids, ITS-2, phylogenetic

Authors

First Name

Hassan

Last Name

Hassanein

MiddleName

A.

Affiliation

Department of Zoology, Faculty of Science, Al-Azhar University, Nasr City 11884, Cairo, Egypt

Email

-

City

Egypt

Orcid

-

First Name

Ayman

Last Name

Elsayed

MiddleName

N.

Affiliation

Department of Zoology, Faculty of Science, Al-Azhar University, Nasr City 11884, Cairo, Egypt

Email

ayman.n.elsayed@azhar.edu.eg

City

Egypt

Orcid

-

First Name

Mohamed

Last Name

Abdelaal

MiddleName

-

Affiliation

Department of Zoology, Faculty of Science, Al-Azhar University, Nasr City 11884, Cairo, Egypt

Email

-

City

Egypt

Orcid

-

First Name

Ahmed

Last Name

Abdel-Aziz

MiddleName

-

Affiliation

Department of Zoology, Faculty of Science, Al-Azhar University, Nasr City 11884, Cairo, Egypt

Email

-

City

Egypt

Orcid

-

Volume

14

Article Issue

2

Related Issue

35558

Issue Date

2022-12-01

Receive Date

2022-09-17

Publish Date

2022-11-27

Page Start

159

Page End

170

Print ISSN

2090-0783

Online ISSN

2090-0856

Link

https://eajbse.journals.ekb.eg/article_272456.html

Detail API

https://eajbse.journals.ekb.eg/service?article_code=272456

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272,456

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Original Article

Type Code

687

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology

Publication Link

https://eajbse.journals.ekb.eg/

MainTitle

Morphological and Molecular Characterization Based on ITS-2 of Moniezia expansa Rudolphi, 1810 (Anoplocephalidae) Isolated from The Intestine of Sheep, Ovis aries (Bovidae) from Egypt

Details

Type

Article

Created At

22 Jan 2023