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209694

Mycobacterium tuberculosis RpfE Protein Purified from H37Rv Culture Filtrate Proteins Stimulates Splenocytes

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Last updated: 22 Jan 2023

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Abstract

Resuscitation promoting factor E (RpfE) proteins in Mycobacterium tuberculosis (M. tuberculosis) may be recognized by the host immune system owing to their secretory nature. In this study, the RpfE protein was purified using DEAE–Sepharose column chromatography from M. tuberculosis H37Rv extracellular proteins. An upregulated production of gamma interferon (IFN- γ), interleukin-10 (IL-10) and IL-12 by splenocytes suggest T cell proliferation stimulated by purified RpfE protein. The present findings imply that the RpfE protein produced extracellularly in M. tuberculosis H37Rv can be used as a viable subunit vaccine candidate.

DOI

10.21608/eajbse.2021.209694

Keywords

Mycobacterium tuberculosis, RpfE, Purification, culture filtrate proteins, Splenocytes

Authors

First Name

Mansoor

Last Name

Alsahag

MiddleName

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Affiliation

Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Albaha University, Albaha, Saudi Arabia

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Volume

13

Article Issue

2

Related Issue

29086

Issue Date

2021-12-01

Receive Date

2021-11-06

Publish Date

2021-12-15

Page Start

35

Page End

44

Print ISSN

2090-0783

Online ISSN

2090-0856

Article File

EAJBSE_Volume 13_Issue 2_Pages 35-44.pdf

PDF . 612.7kB

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https://eajbse.journals.ekb.eg/article_209694.html

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https://eajbse.journals.ekb.eg/service?article_code=209694

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Original Article

Type Code

687

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology

Publication Link

https://eajbse.journals.ekb.eg/

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Article

Created At

22 Jan 2023