To shed some light on distribution, abundance and diversity of flies' species in Wadi El‐Rayan Protected Area, several field trips for one year from winter 2017 to autumn 2018 were carried out. In the present result, the flies in Wadi El‐Rayan Protected Area were comprised 5families include, Calliphoridae, Muscidae, Sarcophagidae, Piophilidae and Phoridae. Calliphoridae was the most abundant and diversify family during the study period, it represented (52.48% of the total flies count) and comprised 5 species (41.67% of the total recorded species), followed by Muscidae (41 Trap/day, 42.88% and 3 species, 25%), Sarcophagidae (2.6 Trap/day, 2.68% and 2 species, 16.67%), Phoridae (1 Trap/day, 1.046% and only species, 8.33%) and Piophilidae with an annual average of (0.88 Trap/day, 0.92% and only one species, 8.33%).But the most dominant flies species were Musca domestica and Chrysomya megacephala. Seasonally, flies were flourished in summer, while winter is the lowest abundant season. Spatially, they regularly distributed among study sites. The previous findings were confirmed by that the flies were semi-stable in their temporal distribution and there is fairness in their spatial distribution. The diversity indices indicate that poor in richness of flies diversity in the investigated area.