Attempts were made to identify, Anopheline species by using Polymerase Chain Reaction (PCR) and to determine the sporozoite infection rate in malaria vectors using Enzyme Linked Immunosorbent Assay (ELISA). Samples were collected from highly endemic areas in central Sudan (Sennar), and eastern Sudan (Koka).Morphological Identification was carried using available keys.  Anopheles gambiae complexwas then identified using PCR.Out of fifty two was identified   as Anopheles arabiensis (92.3 %). The sporozoite infection rate was estimated using ELISA method the infectivity rates were 16% (4/24), 15% (3/21) in Sennar and Koka respectively. This data indicated that the two areas are endemic for malaria and An. arabiensis is the main vector in the study areas. The sporozoite infection rate was found to be 16% (4/24) in Sennar samples, whereas it was found to be 15% (3/21) in Koka samples. Thus, data indicate of these two areas are endemic for malaria and An.arabiensis is the main vector in these areas.